Lactate Dehydrogenase (LDH) Activity Assay Kit
Référence E-BC-K046-S-100
Conditionnement : 100Assays
Marque : Elabscience
| Detection Principle | Coenzyme I as a hydrogen carrier, LDH catalyzes the production of pyruvic acid from lactic acid, pyruvic acid react with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone which is brownish red in alkaline solution. The depth of the color is proportional to the concentration of pyruvic acid. The activity of LDH can be measured indirectly by measuring the OD value at 450 nm. |
| Synonyms | LDH |
| Sample Type | Serum,Plasma,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (450 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Liver And Renal Function , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 4 U/L |
| Detection Range | 4-400 U/L |
| Precision | inter-assay CV: 7.2% | intra-assay CV: 2.2% |
| Sample Volume | 400 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 10-15 |
| Human plasma | 10-15 |
| Porcine serum | 20-30 |
| Rat serum | 20-30 |
| Rat plasma | 20-30 |
| 10% Rat liver tissue homogenate | 800-1000 |
| 10% Mouse kidney tissue homogenate | 500-800 |
| 10% Rat lung tissue homogenate | 300-500 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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