Latrophilin 1 antibody [HL3853]

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Latrophilin 1 antibody [HL3853] (GTX642087) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled IMR-32 whole cell and membrane extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Latrophilin 1 antibody [HL3853] (GTX642087) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)