Images
Flow Cytometry
Application

Flow Cytometry

Flow cytometric analysis of Ramos cells unstained untreated cells as negative control (blue) or stained untreated (red) or treated with pervanadate (green) using LCP2 (phospho Y128) monoclonal antibody (FITC).

  • Specification

    Product Description

    Rabbit monoclonal antibody raised against synthetic phosphopeptide of human LCP2.

    Immunogen

    A synthetic phosphopeptide corresponding to residues surrounding Y128 of human LCP2.

    Host

    Rabbit

    Reactivity

    Human

    Form

    Liquid

    Conjugation

    FITC

    Purification

    Protein A/G Purification

    Isotype

    IgG1k

    Recommend Usage

    Flow Cytometry (5 uL/106 cells or 0.05 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.4 (0.2% BSA, 0.09% sodium azide).

    Storage Instruction

    Store at 2-8°C.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Flow Cytometry

    Flow cytometric analysis of Ramos cells unstained untreated cells as negative control (blue) or stained untreated (red) or treated with pervanadate (green) using LCP2 (phospho Y128) monoclonal antibody (FITC).
  • Gene Info — LCP2

    Entrez GeneID

    3937

    Gene Name

    LCP2

    Gene Alias

    SLP-76, SLP76

    Gene Description

    lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)

    Omim ID

    601603

    Gene Ontology

    Hyperlink

    Gene Summary

    SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function. [provided by RefSeq

    Other Designations

    76 kDa tyrosine phosphoprotein|SH2 domain-containing leukocyte protein of 76kD|lymphocyte cytosolic protein 2|lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of 76kD)

  • Interactome
  • Pathway
  • Disease