LDHB ELISA Kit (Mouse)
Référence OKCD02683
Conditionnement : 96Wells
Marque : Aviva Systems Biology
LDHB ELISA Kit (Mouse) (OKCD02683)
| Datasheets/Manuals | Printable datasheet for LDHB ELISA Kit (Mouse) (OKCD02683) |
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| Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactate Dehydrogenase B (LDHB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactate Dehydrogenase B (LDHB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactate Dehydrogenase B (LDHB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lactate Dehydrogenase B (LDHB) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactate Dehydrogenase B (LDHB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactate Dehydrogenase B (LDHB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | -20°C|2°C to 8°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.055 ng/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of Lactate Dehydrogenase B (LDHB). No significant cross-reactivity or interference between Lactate Dehydrogenase B (LDHB) and analogues was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
| Protocol Information |
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| Gene Symbol | Ldhb |
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| Gene Full Name | lactate dehydrogenase B |
| Alias Symbols | AI790582, H-Ld, H-Ldh, L-lactate dehydrogenase B chain, lactate dehydrogenase 2, B chain, LDH heart subunit, Ldh-, Ldh-2, LDH-B, LDH-H, Ldh2. |
| NCBI Gene Id | 16832 |
| Protein Name | L-lactate dehydrogenase B chain |
| Description of Target | L-lactate dehydrogenase B chain |
| Uniprot ID | P16125 |
| Protein Accession # | NP_001289694.1 |
| Nucleotide Accession # | NM_001302765.1 |
| Protein Size (# AA) | 334 |
