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> Lipoteichoic Acid (LTA) Mouse Monoclonal Antibody [Clone ID: 55]

Lipoteichoic Acid (LTA) Mouse Monoclonal Antibody [Clone ID: 55]

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Référence AM26274LE-N

Conditionnement : 200ug


Lipoteichoic Acid (LTA) Mouse Monoclonal Antibody [Clone ID: 55]

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SKU
AM26274LE-N
Lipoteichoic Acid (LTA) mouse monoclonal antibody, clone 55, Low Endotoxin
Size:
200 ug
Conjugation:
Unconjugated
 

View other "LTA" antibodies

Specifications
Product Data
Clone Name 55
Application ELISA, IF, IHC, WB
Recommended Dilution Immunohistochemistry on Frozen Sections: The typical starting working dilution is 1/50.
Flow Cytometry: 1/250 (Ref.10).
Immunoassay: For detection, 1.2 µg/ml antibody in PBS was added for 1 hr at 37°C on LTA coated plates (Ref.2,3,8).
Immunofluorescence (Ref.4,9): 60' in PBS/0.02%BSA/0.02% Saponin (Ref.4).
Western blot (Ref 5-7): A reduced or native sample treatment and run on 15% SDS-Page.
Blot was incubated o/n at 4°C with a 1/1000 dilution. The band size is ~17 kDa (Ref.6,7).
The typical starting working dilution is 1/50.
Positive Control: Culture medium of Gram-positive bacteria.
Negative Control: Culture medium of eukaryotic cells.
Reactivity Streptococcus sobrims
Antibody Host Mouse
Isotype IgG3
Clonality Monoclonal
Immunogen Microbial mixture of Streptococcus sobrims HG961, HG962, HG970, and HG977 (Ref.1).
Specificity

The monoclonal antibody 55 recognizes Lipoteichoic Acid (LTA).
Buffer State: Low Endotoxin
State: Liquid Culture Medium with a Low Endotoxin level
Preservative: 0.02% Sodium Azide
Concentration lot specific
Conjugation Unconjugated
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Background LTA, a glycerol phosphate surface polymer, is a component of the envelope of Gram-positive bacteria. LTA is anchored via its glycolipids to the membrane and carries a polysaccharide chain extending into the peptidoglycan layer of the cell wall. LTA is released spontaneously into the culture medium during growth of gram-positive bacteria. LTA functions as an immune activator with characteristics very similar to lipopolysaccharide (LPS) from Gram-negative bacteria. LTA binds to CD14 and triggers activation predominantly via Toll-like receptor 2. Although LTA is internalized and traffics to the Golgi, the cellular activation in response to LTA occurs at the cell surface.
Reference Data
Product Manuals
FAQs
  • Antibody and Western Standard
SDS

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