Specifications

Product Data
Applications	IHC
Recommended Dilution	IHC: 50-200 Positive control: Human liver cancer Predicted cell location: Cytoplasm, Nucleus
Reactivities	Human, Mouse, Rat
Host	Rabbit
Isotype	IgG
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to a region derived from 370-383 amino acids of Human SMAD family member 7
Formulation	PBS pH7.3, 0.05% NaN3, 50% glycerol
Concentration	lot specific
Purification	Antigen affinity purification
Conjugation	Unconjugated
Storage	Store at -20°C as received.
Stability	Stable for 12 months from date of receipt.
Gene Name	SMAD family member 7
Database Link	NP_005895 Entrez Gene 17131 MouseEntrez Gene 81516 RatEntrez Gene 4092 Human UniProt ID O15105
Background	The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding; this complex translocates to the cytoplasm; where it interacts with TGF-beta receptor type-1 (TGFBR1); leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene.
Synonyms	CRCS3; MADH7; MADH8
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	TGF-beta signaling pathway