Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

MIF, recombinant Human

Imprimer

Référence NB-50-0369-25

Conditionnement : 25µg

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Téléphone : +1 850 650 7790

Marque : Neo Biotech

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Contactez votre distributeur local :

Téléphone : +1 850 650 7790

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Bioassay analysis of Human MIF Recombinant Protein, PeproTech® (Product # 300-69-1MG).

Product Details

300-69-100UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species

Human

Published species

Human, Mouse

Expression System

Insect cells

Amino acid sequence

HHHHHHHHAM PMFIVNTNVP RASVPDGFLS ELTQQLAQAT GKPPQYIAVH VVPDQLMAFG GSSEPCALCS LHSIGKIGGA QNRSYSKLLC GLLAERLRIS PDRVYINYYD MNAANVGWNN STFA

Tag

His-tag

Molecular weight

15 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to inhibit monocyte migration.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Insect cell-derived Recombinant Human MIF is a 15 kDa protein containing 124 amino acid residues, including an N-terminal His-tag.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Rapid generation of monocyte-derived antigen-presenting cells with dendritic cell-like properties.

Transfusion

Cunningham S,Hackstein H

Tue Jun 01 00:00:00 UTC 2021

Single-Cell Analyses Inform Mechanisms of Myeloid-Targeted Therapies in Colon Cancer.

Cell

Zhang L,Li Z,Skrzypczynska KM,Fang Q,Zhang W,O'Brien SA,He Y,Wang L,Zhang Q,Kim A,Gao R,Orf J,Wang T,Sawant D,Kang J,Bhatt D,Lu D,Li CM,Rapaport AS,Perez K,Ye Y,Wang S,Hu X,Ren X,Ouyang W,Shen Z,Egen JG,Zhang Z,Yu X

Thu Apr 16 00:00:00 UTC 2020

CXCR4 Signaling Has a CXCL12-Independent Essential Role in Murine MLL-AF9-Driven Acute Myeloid Leukemia.

Cell reports

Ramakrishnan R,Peña-Martínez P,Agarwal P,Rodriguez-Zabala M,Chapellier M,Högberg C,Eriksson M,Yudovich D,Shah M,Ehinger M,Nilsson B,Larsson J,Hagström-Andersson A,Ebert BL,Bhatia R,Järås M

300-69 was used in Sample Preparation (FC/FACS) to identify CXCR4 signaling as essential for AML stem cells by protecting them from differentiation independent of CXCL12 stimulation.

Tue May 26 00:00:00 UTC 2020

The role of macrophage migration inhibitory factor in promoting benign prostatic hyperplasia epithelial cell growth by modulating COX-2 and P53 signaling.

Biology open

Song H,Shen Q,Hu S,Jin J

300-69 was used in In vitro experiments to study whether the proinflammatory cytokine macrophage migration inhibitory factor (MIF) has a role in benign prostatic hyperplasia (BPH) progression.

Thu Nov 12 00:00:00 UTC 2020

Growth Factor-like Gene Regulation Is Separable from Survival and Maturation in Antibody-Secreting Cells.

Journal of immunology (Baltimore, Md. : 1950)

Stephenson S,Care MA,Fan I,Zougman A,Westhead DR,Doody GM,Tooze RM

Fri Feb 15 00:00:00 UTC 2019

Macrophage Migration Inhibitory Factor Acts as the Potential Target of a Newly Synthesized Compound, 1-(9'-methyl-3'-carbazole)-3, 4-dihydro-β-carboline.

Scientific reports

Ko PH,Shen YC,Murugan K,Huang CW,Sivakumar G,Pal P,Liao CC,Luo KS,Chuang EY,Tsai MH,Lai LC

Thu Feb 14 00:00:00 UTC 2019

Macrophage migration inhibitory factor downregulation: a novel mechanism of resistance to anti-angiogenic therapy.

Oncogene

Castro BA,Flanigan P,Jahangiri A,Hoffman D,Chen W,Kuang R,De Lay M,Yagnik G,Wagner JR,Mascharak S,Sidorov M,Shrivastav S,Kohanbash G,Okada H,Aghi MK

300-69 was used in Cell Culture to study the mechanisms of resistance to anti-angiogenic therapies for cancer such as VEGF neutralizing antibody bevacizumab to show that bevacizumab resistance is driven by reduced migration inhibitory factor (MIF) at the tumor edge causing proliferative expansion of M2 macrophages, which in turn promotes tumor growth.

Thu Jun 29 00:00:00 UTC 2017

Macrophage migration inhibitory factor-CXCR4 is the dominant chemotactic axis in human mesenchymal stem cell recruitment to tumors.

Journal of immunology (Baltimore, Md. : 1950)

Lourenco S,Teixeira VH,Kalber T,Jose RJ,Floto RA,Janes SM

Wed Apr 01 00:00:00 UTC 2015

Potent Paracrine Effects of human induced Pluripotent Stem Cell-derived Mesenchymal Stem Cells Attenuate Doxorubicin-induced Cardiomyopathy.

Scientific reports

Zhang Y,Liang X,Liao S,Wang W,Wang J,Li X,Ding Y,Liang Y,Gao F,Yang M,Fu Q,Xu A,Chai YH,He J,Tse HF,Lian Q

300-69 was used in In vivo experiments to demonstrate that macrophage migration inhibitory factor (MIF) and growth differentiation factor-15 (GDF-15) in conditioned medium harvested from induced pluripotent stem cell-derived mesenchymal stem cells (iPSC-MSCs-CdM), provide enhanced cardioprotection against anthracycline-induced cardiomyopathy compared to conditioned medium harvested from bone marrow mesenchymal stem cells (BM-MSCs-CdM).

Tue Jun 09 00:00:00 UTC 2015