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> Monoolein [111-03-5]

Monoolein [111-03-5]

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Référence HY-128754-25mg

Conditionnement : 25mg

Marque : MedChemExpress


Description

Monoolein is a biocompatible lipid molecule that can be used as a carrier for bone repair. Monoolein exhibits anti-inflammatory activity by inhibiting the immune response induced by LPS (HY-D1056). It exerts its anti-inflammatory effects by reducing the production of immune response factors such as IL-12 p40, IL-6, and TNF-α, and inhibiting the generation of NO. Monoolein can be used in drug delivery and research in the field of inflammatory diseases [1][2].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Monoolein (1-10 μM, 1 hour) exerts anti-inflammatory effects in BMDCs and RAW264.7 cells by inhibiting LPS-induced immune responses, manifested as a reduction in IL-12 p40, IL-6, and TNF-α levels in BMDCs, and suppression of NO, IL-12 p40, IL-6, and TNF-α production in RAW264.7 cells [1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Monoolein Related Antibodies

Western Blot Analysis[1]

Cell Line: Bone marrow-derived dendritic cells (BMDCs)
Concentration: 1-10 μM
Incubation Time: 1 h
Result: Significantly reduced the levels of IL-12 p40, IL-6, and TNF-α in BMDCs.
In Vivo

Monoolein (carrying 5 μg rhBMP-2, applied to the bone defect area for 3 weeks and 6 weeks) can serve as a carrier for rhBMP-2, playing a role in bone repair and demonstrating good biocompatibility and protein release properties [2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Wistar rats (200 g)
Dosage: Monoolein carrier with rhBMP-2, 5 μg of rhBMP-2 per rat
Administration: 3 weeks or 6 weeks, single dose, Monoolein combined with rhBMP-2 applied to bone defect site
Result: Promoted new bone formation, with better repair at 6 weeks compared to 3 weeks.
Masse moléculaire

356.54

Formule

C21H40O4
CAS No.
Appearance

Oil

Color

White to light yellow

SMILES

CCCCCCCC/C=C\CCCCCCCC(OCC(O)CO)=O
Structure Classification
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvant et solubilité
In Vitro: 

Ethanol : 100 mg/mL (280.47 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8047 mL 14.0237 mL 28.0473 mL
5 mM 0.5609 mL 2.8047 mL 5.6095 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.01 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% EtOH    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (7.01 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Pureté et documentation
Références