Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> Mouse Anti-Human IgD-HRP

Mouse Anti-Human IgD-HRP

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Référence 9030-05

Conditionnement : 1.0mL

Marque : Southern Biotech

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Téléphone : +1 850 650 7790

Product Specifications

More Information
Clone IADB6
Isotype Mouse IgG2aκ
Isotype Control Mouse IgG2a-HRP (HOPC-1)
Specificity Human IgD
Immunogen Human IgD myeloma protein
Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Clonality Monoclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications ELISA – Quality tested
FLISA – Quality tested
Flow Cytometry – Quality tested 4-10
Immunohistochemistry-Frozen Sections – Reported in literature 2
Immunohistochemistry-Paraffin Sections – Reported in literature 3
Immunoprecipitation – Reported in literature 1
Separation – Reported in literature 11,12
Costimulation – Reported in literature 13,14

RRID Number AB_2796593
Gene ID 3495 (Human)
Gene ID Symbol IGHD (Human)
UniProt ID P01880 (Human
UniProt Name IGHD_HUMAN (Human)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view

Other Conjugates and Formulations

UNLB FITC AP BIOT PE AF488 AF647

Citations

  1. 1. Kiyotaki M, Cooper MD, Bertoli LF, Kearney JF, Kubagawa H. Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells. J Immunol. 1987;138:4150-8. (IP)
  2. 2. Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, et al. IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells. J Immunol. 2007;178:2872-82. (IHC-FS)
  3. 3. Shi S, Key ME, Kalra KL. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem. 1991;39:741-8. (IHC-PS)
  4. 4. Klein U, Küppers R, Rajewsky K. Variable region gene analysis of B cell subsets derived from a 4-year-old child: somatically mutated memory B cells accumulate in the peripheral blood already at young age. J Exp Med. 1994;180:1383-93. (FC)
  5. 5. Krzysiek R, Lefèvre EA, Zou W, Foussat A, Bernard J, Portier A, et al. Antigen receptor engagement selectively induces macrophage inflammatory protein-1α (MIP-1α) and MIP-1β chemokine production in human B cells. J Immunol. 1999;162:4455-63. (FC)
  6. 6. Jaimes MC, Rojas OL, Kunkel EJ, Lazarus NH, Soler D, Butcher EC, et al. Maturation and trafficking markers on rotavirus-specific B cells during acute infection and convalescence in children. J Virol. 2004;78:10967-76. (FC)
  7. 7. He B, Xu W, Santini PA, Polydorides AD, Chiu A, Estrella J, et al. Intestinal bacteria trigger T cell-independent immunoglobulin A2 class switching by inducing epithelial-cell secretion of the cytokine APRIL. Immunity. 2007;26:812-26. (FC)
  8. 8. Rojas OL, Caicedo L, Guzmán C, Rodríguez L, Castañeda J, Uribe L, et al. Evaluation of circulating intestinally committed memory B cells in children vaccinated with attenuated human rotavirus vaccine. Viral Immunol. 2007;20:300-11. (FC)
  9. 9. Smith K, Garman L, Wrammert J, Zheng N, Capra JD, Ahmed R, et al. Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen. Nature Protoc. 2009;4:372-84. (FC)
  10. 10. Suryani S, Fulcher DA, Santner-Nanan B, Nanan R, Wong M, Shaw PJ, et al. Differential expression of CD21 identifies developmentally and functionally distinct subsets of human transitional B cells. Blood. 2010;115:519-29. (FC)
  11. 11. Al-Darmaki S, Knightshead K, Ishihara Y, Best A, Schenkein HA, Tew JG, et al. Delineation of the role of platelet-activating factor in the immunoglobulin G2 antibody response. Clin Diagn Lab Immunol. 2004;11:720-8. (Sep)
  12. 12. Xu W, Santini PA, Matthews AJ, Chiu A, Plebani A, He B, et al. Viral double-stranded RNA triggers Ig class switching by activating upper respiratory mucosa B cells through an innate TLR3 pathway involving BAFF. J Immunol. 2008;181:276-87. (Sep)
  13. 13. Kuritani T, Cooper MD. Human B cell differentiation. III. Enhancing effect of monoclonal anti-immunoglobulin D antibody on pokeweed mitogen-induced plasma cell differentiation. J Immunol. 1982;129:2490-5. (Costim)
  14. 14. Igarashi H, Sakaguchi N. Telomerase activity is induced in human peripheral B lymphocytes by the stimulation to antigen receptor. Blood. 1997;89:1299-1307. (Costim)