Quantibody® Mouse Cytokine Array 5 Kit. Detects 40 Mouse Cytokines. Suitable for all liquid sample types.
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Mouse Cytokine Array Q5
Mouse Cytokine Array Q5
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 40 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
bFGF
| BLC (CXCL13)
| CD30 Ligand (TNFSF8)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
|
Fas Ligand (TNFSF6)
| GCSF
| GM-CSF
| ICAM-1 (CD54)
| IFN-gamma
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
| IL-3
| IL-4
|
IL-5
| IL-6
| IL-7
| IL-10
| IL-12 p40
|
IL-13
| IL-15
| IL-17A
| IL-21
| KC (CXCL1)
|
Leptin
| LIX
| MCP-1 (CCL2)
| MCP-5
| M-CSF
|
MIG (CXCL9)
| MIP-1 alpha (CCL3)
| MIP-1 gamma
| Platelet Factor 4 (CXCL4)
| RANTES (CCL5)
|
TARC (CCL17)
| I-309 (TCA-3/CCL1)
| TNF alpha
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Mouse Cytokine Array Q5 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAM-CYT-5.gal)
Excel (QAM-CYT-5_Analysis_Tool.xls)
citations
8-oxo-7,8-dihydroguanosine (8-oxo-Guo) drives pulmonary inflammatory pathways through pattern recognition receptors
- Year:
- 2026
- Journal Name:
- Redox Biology
- PubMed ID:
- 42190561
A low-toxicity circular single-stranded DNA platform for safe and potent IL-12 immunotherapy against hepatocellular carcinoma
- Year:
- 2026
- Journal Name:
- Journal of Nanobiotechnology
- PubMed ID:
- 41896926
Secretome Profiling of Young Multipotent Stem Cells Reveals Angiogenic and Immunomodulatory Mechanisms Supporting Aged Neuromuscular Health
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (culture media from young, old MDSPCs)
- Year:
- 2026
- Journal Name:
- Aging Cell
- PubMed ID:
- 41674450
HPV16-Expressing Tumors Release Multiple IL1 Ligands to Orchestrate Systemic Immunosuppression Whose Disruption Enables Efficacy of a Therapeutic Vaccine
- Species:
- Mouse
- Sample Type:
- Serum (K14HPV16/H2b vs FVBN/H2b mice)
- Year:
- 2025
- Journal Name:
- Cancer Discovery
- PubMed ID:
- 40287949
Suppression of multiple mouse models of refractory malignancies by reprogramming IL-18 ligand-receptor interaction
- Year:
- 2025
- Journal Name:
- Nature Communications
- PubMed ID:
- 40610476
The Bacterial Outer Membrane Vesicle-Cloaked Immunostimulatory Nanoplatform Reinvigorates T Cell Function and Reprograms Tumor Immunity
- Species:
- Mouse
- Sample Type:
- Serum (4T1 tumor-bearing BALB/c mice)
- Year:
- 2025
- Journal Name:
- ACS Nano
- PubMed ID:
- 40392526
Earlier onset of chemotherapy-induced neuropathic pain in females by ICAM-1-mediated accumulation of perivascular macrophages
- Species:
- Mouse
- Sample Type:
- Other / Unspecified (spinal cord dorsal horn samples)
- Year:
- 2025
- Journal Name:
- Science Advances
- PubMed ID:
- 40238872
Chronic inflammation deters natural killer cell fitness and cytotoxicity in myeloid leukemia
- Year:
- 2025
- Journal Name:
- Blood Advances
- PubMed ID:
- 39571169
Bone marrow mesenchymal stem cells derived cytokines associated with AKT/IAPs signaling ameliorate Alzheimer's disease development
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (conditioned medium from mBMMSCs)
- Year:
- 2025
- Journal Name:
- Stem Cell Research & Therapy
- PubMed ID:
- 39849525
Targeting glutamine catabolism suppresses eosinophil expansion and activation to alleviate allergic airway inflammation
- Year:
- 2025
- Journal Name:
- Biochemical Pharmacology
- PubMed ID:
- 41125213
