Mouse eosinophil cationic protein,ECP ELISA Kit
Référence CSB-E11799m-24T
Conditionnement : 24T
Marque : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
eosinophil cationic protein,ECP
Abbreviation
ECP
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ECP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-106 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-99 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 83-95 | |||||
Recovery
| The recovery of mouse ECP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
| EDTA plasma (n=4) | 95 | 89-99 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Mouse ECP ELISA Kit was designed for the quantitative measurement of Mouse ECP protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .
Citations
- U Park, JH Lee, U Kim, K Jeon, Y Kim,Journal of Medical Virology,2024
- Z Wang,Journal of asthma and allergy,2024
- JH Jo,The World Allergy Organization journal,2024
- S Wu,International immunopharmacology,2023
- SN Saxton,American Journal of Physiology-Heart and Circulatory Physiology,2020
- J Li,Molecular Immunology,2020
- Xu C, et al,Faseb Journal,2019
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