Amount Used: ?1 ug/106 cells Human peripheral blood mononuclear cells were labeled with either mouse anti-human CD3 (IgG1) (A), anti-human CD5 (IgG2a) (B), anti-human CD22 (IgG2b) (C), anti-human IgD (IgG3) (D) or anti-human CD57 (IgM) (E). After washing the cells were then stained with goat anti-mouse IgG2b-FITC, following which lymphocytes were gated and analyzed by flow cytometry.
Liquid 50mM Tris/1mM MgCl2 and 50% glycerol (pH 8.0) containing sodium azide as preservative
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Conjugation
AP
Application
IHC-F, IHC-P, ICC, WB, ELISPOT
Reconstitution and Storage
Store at 2-8°C or long-term at -20°C.
Immunogen
Mouse IgG2b
Purification
Affinity chromatography on mouse IgG2b covalently linked to agarose
Specificity
Reacts with the heavy chain of mouse IgG2b. Mouse IgG1, IgG2a, IgG3, IgM and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species.
Source
Pooled antisera from goats hyperimmunized with mouse IgG2b
Characterization
To insure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry for conformance to characteristics of a standard reference reagent.
Warning
Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Application Info
ELISA: 1:2000~4000
Other Applications Data
Since applications vary, you should determine the optimum working dilution ofthe product that is appropriate for your specific need.
Cross Absorption
Mouse IgM, IgG1, IgG2a, IgG3 and IgA, pooled human sera and purified humanparaproteins.
What is the species homology for "Goat Anti-Mouse IgG2b Antibody - AP Conjugated (OASB01607)"?
The tested species reactivity for this item is "Mouse". This antibody is predicted to have homology to "Mouse".
How long will it take to receive "Goat Anti-Mouse IgG2b Antibody - AP Conjugated (OASB01607)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
What buffer format is "Goat Anti-Mouse IgG2b Antibody - AP Conjugated (OASB01607)" provided in?
This item is provided in "Liquid 50mM Tris/1mM MgCl2 and 50% glycerol (pH 8.0) containing sodium azide as preservative ". Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "Goat Anti-Mouse IgG2b Antibody - AP Conjugated (OASB01607)"?
This target may also be called "IgG2, IgG2b, Igh-3, gamma2b" in publications.
What is the shipping cost for "Goat Anti-Mouse IgG2b Antibody - AP Conjugated (OASB01607)"?
The shipping cost for this item is within the US. Please contact us for specific shipping for international orders.
What is the guarantee for "Goat Anti-Mouse IgG2b Antibody - AP Conjugated (OASB01607)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "Goat Anti-Mouse IgG2b Antibody - AP Conjugated (OASB01607)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "". Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "Goat Anti-Mouse IgG2b Antibody - AP Conjugated (OASB01607)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "IGHG2B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "IGHG2B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "IGHG2B"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "IGHG2B"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "IGHG2B"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "IGHG2B"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
Format: The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added.
Size: 1ML
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Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunocytochemistry|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot
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