Mouse IgG3 isotype Control Antibody

Référence orb343779-1mg

Conditionnement : 1mg

Marque : Biorbyt


Mouse IgG3 isotype Control Antibody

SKU: orb343779

Description

Mouse IgG3 isotype Control Antibody

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsELISA, FC, SDS-PAGE
Dilution RangeFC: 1:1000-1:5000
Application Notes
Mouse IgG3 isotype control has been tested by SDS-Page and ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Key Properties

IsotypeIgG3
Clone No.MG3
PurityMouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG3 and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM. Confirmed by SDS-PAGE.
ConjugationUnconjugated

Storage & Handling

StorageStore vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.0 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

MOUSE IgG3 subclass isotype control, MOUSE IgG3 isotype control

Protocol Information

FC
Flow Cytometry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol