Orb43559-0.1mg | Mouse IgG3 Isotype Control Purified Antibody Clinisciences

Mouse IgG3 Isotype Control Purified Antibody

SKU: orb43559

This product can be custom made

Description

Mouse IgG3 Isotype Control

Research Area

Immunology & Inflammation

Images & Validation

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Tested Applications	ELISA, FC, ICC, IHC-Fr, IHC-P, IP, WB
Application Notes	Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.

Tested Applications

ELISA, FC, ICC, IHC-Fr, IHC-P, IP, WB

Application Notes

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.

Key Properties

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Antibody Type	Secondary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG3
Clone No.	PPV-07
Purification	Purified by protein-A affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	HEPES buffered saline (HBS), pH 7.0, 15 mM sodium azide
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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