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> Mucicarmine [51395-97-2]

Mucicarmine [51395-97-2]

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Référence HY-D1542-1g

Conditionnement : 1g

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Mucicarmine is used in the histological visualization of acid mucopolysaccharides in tissue sections. Mucicarmine can identify mucin (deep rose), which is useful in determining the mucin positive cancer (such as liver carcinoma).

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Mucicarmine Chemical Structure

Mucicarmine Chemical Structure

CAS No. : 51395-97-2

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Based on 1 publication(s) in Google Scholar

Description

Mucicarmine is used in the histological visualization of acid mucopolysaccharides in tissue sections. Mucicarmine can identify mucin (deep rose), which is useful in determining the mucin positive cancer (such as liver carcinoma)[1].

IC50 & Target

Mucin[1]
In Vitro

Mucicarmine successfully stains the presence of mucin on frozen-tissue sections (Mucin stained deep rose).
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Mount the cryostat sections (thickness: 4 to 5 μm) onto coated glass slides.
2. Fix the sections in frozen-section fixative immediately for 30 seconds and then rinse with water for 5 seconds.
3. Immerse the glass slides for 30 seconds into Harris hematoxylin, then consecutively into distilled water, ammonium hydroxide, and distilled water, for 5 seconds in each solution.
4. The mucicarmine stain is placed in the microwave oven and microwave until the solution is about to bubble (30 seconds).
5. Immerse the glass slides in the warmed mucicarmine solution for 2 minutes and rinse slides in distilled water for 5 seconds.
7. Immerse the glass slides consecutively in 95% alcohol, 100% alcohol, and xylene for 5 seconds in each solution, then coverslip the slides.
Note: Nuclei stain (blue) and connective tissue stain (green) maybe is necessary according to your specific needs.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Mucicarmine Related Antibodies
CAS No.

51395-97-2
Appearance

Solid

Color

Dark purple to black

SMILES

[Mucicarmine]
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Pureté et documentation
Références

  • [1]. S Soans, et al. Mucin stain on frozen sections: a rapid 3-minute method. Arch Pathol Lab Med. 1999 May;123(5):378-80.  [Content Brief]

  • [1]. S Soans, et al. Mucin stain on frozen sections: a rapid 3-minute method. Arch Pathol Lab Med. 1999 May;123(5):378-80.

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Mucicarmine Related Classifications

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  • Calculateur de dilution

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Mucicarmine51395-97-2Fluorescent DyeMucinliver carcinomamucopolysaccharidedyehistologicalsectionInhibitorinhibitorinhibit