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> Neurofilament, phospho (NF-H) (Neuronal Marker)(NE14), Biotin conjugate, 0.1mg/mL

Neurofilament, phospho (NF-H) (Neuronal Marker)(NE14), Biotin conjugate, 0.1mg/mL

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Référence BNCB1253-500

Conditionnement : 500uL

Marque : Biotium

Neurofilament, phospho (NF-H) (Neuronal Marker)(NE14), Biotin conjugate, 0.1mg/mL

Neurofilament H, phospho Monoclonal Mouse Antibody (NE14)

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Product Description

This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Catalog number key for antibody number 1253, Anti-Neurofilament H, phospho (NE14) 

Antibody # prefixConjugationEx/Em (nm)Laser lineDetection channelDye Features
BNC04CF®405S404/431405DAPI (microscopy), AF405CF®405S Features
BNC88CF®488A490/515488GFP, FITCCF®488A Features
BNC68CF®568562/583532, 561RFP, TRITCCF®568 Features
BNC94CF®594593/614561Texas Red®CF®594 Features
BNC40CF®640R642/662633-640Cy®5CF®640R Features
BNC47CF®647650/665633-640Cy®5CF®647 Features
BNC74CF®740742/767633-685775/50CF®740 Features
BNCBBiotinN/AN/AN/A
BNUBPurifiedN/AN/AN/A
BNUMPurified,
BSA-free		N/AN/AN/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
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Primary & Secondary Antibody Conjugates

Product Attributes

Antibody number
#1253
Antibody reactivity (target)
Neurofilament H, phospho
Antibody type
Primary
Host species
Mouse
Clonality
Monoclonal
Clone
NE14
Isotype
IgG1, kappa
Molecular weight
200 kDa
Synonyms
NEFH; Neurofilament H; Neurofilament Heavy Polypeptide 200kDa; Neurofilament Triplet H Protein; NF-H; NF200
Human gene symbol
NEFH
Entrez gene ID
4744
SwissProt
P12036
Unigene
198760
Immunogen
Crude neurofilament preparation from porcine spinal cord
Verified antibody applications
Flow (intracellular) (verified), IHC (FFPE) (verified), WB (verified)
Antibody target cellular localization
Cytoskeleton
Species reactivity
Cat, Chicken, Cow, Gerbil, Guinea pig, Human, Mouse, Pig, Rabbit, Rat
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunohistochemistry (formalin-fixed): 0.25-0.5 ug/mL for 30 minutes at RT, Western blot: 1-2 ug/mL, Flow cytometry: 0.5-1 ug/million cells, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes, Optimal dilution for a specific application should be determined by user
Positive control
HEK293 cells, Brain, Neuroblastoma.
Shipping condition
Room temperature
Storage Conditions
Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Regulatory status
For research use only (RUO)
Antibody/conjugate formulation
Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Shelf life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Cell/tissue expression
Neuroendocrine cells, Neurons
Product origin
Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Antibody research areas
Cancer, Cytoskeleton, Neuroscience

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