Oct4 (POU5F1) (NM_203289) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence A DNA sequence encoding human full-length POU5F1/OCT4
Tag	N-His
Predicted MW	21.1 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% sarkosyl, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping	Dry Ice
Reference Data
RefSeq	NP_976034
Locus ID	5460
UniProt ID	M1S623
Cytogenetics	6p21.33
RefSeq Size	1155
RefSeq ORF	795
Synonyms	Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4
Summary	This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. provided by RefSeq, Oct 2013
Protein Families	Adult stem cells, Cancer stem cells, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Transcription Factors

FAQs

Protein FAQs

SDS

SDS for TP760938

Recombinant Protein Resources

Video tutorial: Why protein expression host matters Webinar Video: Overexpression lysate as positive controls for Western blot Product Overview Video: Overexpression lysates as positive controls for Western blot Video Tutorial: Knockout(KO) lysate and antibody specificity Product Brochure: Protein

SKU	Description	Size
PH311998	POU5F1 MS Standard C13 and N15-labeled recombinant protein (NP_002692)	10 ug
LC400950	Lyophilized POU5F1 HEK293T cell transient overexpression lysate (as WB positive control)	20 ug
LC404373	Lyophilized POU5F1 HEK293T cell transient overexpression lysate (as WB positive control)	20 ug
LY400950	Transient overexpression lysate of POU class 5 homeobox 1 (POU5F1), transcript variant 1 (as WB positive control)	100 ug
LY404373	Transient overexpression lysate of POU class 5 homeobox 1 (POU5F1), transcript variant 2 (as WB positive control)	100 ug
TP311998	Recombinant protein of human POU class 5 homeobox 1 (POU5F1), transcript variant 1, 20 µg	20 ug