PCR Clean-Up & Gel Extraction BioSeparation™ Kit

The PCR Clean-Up & Gel Extraction BioSeparation™ Kit provides a cost-effective system for the fast and easy isolation of the DNA fragments from PCR reactions, agarose gels, or enzymatic reactions. The DNA fragments (100bp~10Kb) in the special buffers are bound by the glass fiber matrix of the spin column while contaminants pass through the column. Impurities are efficiently washed away, and the pure DNA is eluted with the Tris buffer or water without phenol extraction or alcohol precipitation. The DNA purified with the kits is suitable for any subsequent application, such as ligation and transformation, sequencing, restriction enzyme digestion, labeling, PCR, in vitro transcription, or microinjection. The entire procedure can be completed within 15~20 minutes.

Fastest Procedure: Completed within 30mins
Sample: up to 100ul of PCR Product or 300mg of Agarose Gel
Recovery: up to 95%

Buffer B: 60ml
Buffer W1: 45ml
Buffer W2: 15ml (Add 60 ml of the ethanol (96~100%) to the Buffer W2 before use)
Buffer E: 10ml
PG Columns: 100 pcs
Collection Tubes: 100 pcs

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards