PDGF AB Protein
Référence OPPA00605
Conditionnement : 2ug
Marque : Aviva Systems Biology
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| Datasheets/Manuals | Printable datasheet for OPPA00605 |
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| Predicted Species Reactivity | Human |
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| Product Format | The protein was lyophilized with no additives. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder. |
| Host | E. Coli |
| Additional Information | Solubility: It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-AB in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
| :: | Biological Activity: The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is < 1 ng/ml, corresponding to a Specific Activity of 1 MIU/mg. |
| :: | Product Introduction: The term 'PDGF' refers to a family of disulphide bond-linked dimeric isoforms that act as autocrine and paracrine growth factors and are produced by a variety of cell types other than platelets.They act as potent mitogens for almost all mesenchymally-derived cells. Aberrant expression is involved in certain cancers, fibroproliferative disorders and atherosclerosis. The protein also contributes to wound healing and neural regeneration. There are four members of the PDGF family - PDGF A, PDGF B, PDGF C and PDGF D. Two distinct types of PDGF-A exist - a short form that is soluble and a long form that is retained by the extracellular matrix. Product Description: Platelet-Derived Growth Factor AB Human Recombinant is a heterodimeric, non-glycosylated, polypeptide chain containing 234 amino acids consisting of 13.3kDa alpha-chain and 12.2 beta-chain having a total molecular mass of 25.5kDa. PDGF-AB is purified by proprietary chromatographic techniques. |
| Reconstitution and Storage | Lyophilized Platelet-derived Growth Factor AB although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution PDGF-AB should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity | Greater than 97.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Peptide Sequence | The sequence of the first five N - terminal amino acids was determined and was found to be Met - Ser - Ile - Glu - Glu - alpha chain and Met - Ser - Leu - Gly - Ser - beta chain. |
| Gene Symbol | PDGF AB |
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| Alias Symbols | pdgf, pdgfa, pdgf-a |
| NCBI Gene Id | 397765 |
| Protein Name | Platelet-derived growth factor subunit A |
| Description of Target | Recombinant Human Platelet Derived Growth Factor-AB |
| Uniprot ID | P13698 |
| Protein Accession # | NP_001081304.1 |
| Protein Size (# AA) | Recombinant |