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> PEDF Protein, Human, Recombinant

PEDF Protein, Human, Recombinant

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Référence NB-64-128253-1mg

Conditionnement : 1mg

Marque : Neo Biotech


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PEDF Protein, Human, Recombinant

(Synonyms: SERPINF1, serpin family F member 1, Pigment epithelium-derived factor, PIG35, PEDF, OI6, OI12, EPC-1) Copy Product Info

Synonyms: SERPINF1, serpin family F member 1, Pigment epithelium-derived factor, PIG35, PEDF, OI6, OI12, EPC-1

Catalog No. TMPS-00148 Copy Product Info
PEDF Protein, Human, Recombinant is expressed in E. coli. The accession number is P36955.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Bioactivity
Fully biologically active when compared to standard. The ED 50 as determined by its ability to enhance the adhesion of human Saos2 cells to bovine Collagen I coated plate is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 10 5 IU/mg.
Description
PEDF Protein, Human, Recombinant is expressed in E. coli. The accession number is P36955.
Species
Human
Expression System
E. coli
TagTag Free
Accession NumberP36955
ConstructionGln20-Pro418
Protein Purity
> 97% as determined by SDS-PAGE; > 97% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a 0.2 μm filtered solution in 20 mM PB, pH 7.4, 150 mM NaCl.
ReconstitutionIt is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.
SynonymsSERPINF1, serpin family F member 1, Pigment epithelium-derived factor, PIG35, PEDF, OI6, OI12, EPC-1
Research Background
PEDF is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. It is a 50 kDa glycoprotein produced and secreted in many tissues throughout the body. A major component of the anti-angiogenic action of PEDF is the induction of apoptosis in proliferating endothelial cells. In addition, PEDF is able to inhibit the activity of angiogenic factors such as VEGF and FGF-2. The neuroprotective effects of PEDF are achieved through suppression of neuronal apoptosis induced by peroxide, glutamate, or other neurotoxins. The recent identification of a lipase-linked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity should facilitate further elucidation of the underlying mechanisms of this pluripotent serpin. To date, PEDF-R is the only signaling receptor known to be used by a serpin family member. The unique range of PEDF activities implicate it as a potential therapeutic agent for the treatment of vasculature related neurodegenerative diseases such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR). PEDF also has the potential to be useful in the treatment of various angiogenesis-related diseases including a number of cancers.
Chemical Properties
Molecular Weight44.4 kDa (Predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageUpon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles.

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Related Tags: PEDF Protein, Human, Recombinant chemical structure | PEDF Protein, Human, Recombinant molecular weight