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> PEDF Protein, Rat, Recombinant (His)

PEDF Protein, Rat, Recombinant (His)

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Référence NB-64-55496-50ug

Conditionnement : 50ug

Marque : Neo Biotech


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PEDF Protein, Rat, Recombinant (His)

(Synonyms: serpin peptidase inhibitor, clade F，member 1) Copy Product Info

Synonyms: serpin peptidase inhibitor, clade F，member 1

Catalog No. TMPY-00266 Copy Product Info
PEDF Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.9 kDa and the accession number is Q68G45.
Other Forms of PEDF Protein, Rat, Recombinant (His):
PEDF Protein, Mouse, Recombinant (His)
PEDF Protein, Human, Recombinant (His)
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Bioactivity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
PEDF Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.9 kDa and the accession number is Q68G45.
Species
Rat
Expression System
HEK293 Cells
TagC-His
Accession NumberQ68G45
ConstructionA DNA sequence encoding the rat Serpinf1 (NP_808788.1) (Met1-Thr418) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Gln 20
Protein Purity
> 90 % as determined by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonymsserpin peptidase inhibitor, clade F，member 1
Research Background
Pigment epithelium-derived factor, also known as PEDF, Serpin F1, and SERPINF1, is a multiple functional protein that has both anti-angiogenic activity and neurotrophic activity at the same time. PEDF is a secreted glycoprotein that belongs to the noninhibitory serpin. It has an alpha/beta core serine-protease inhibitor domain, three major beta-sheets, and ten alpha-helices. PEDF does not inhibit either serine or cysteine proteinases. PEDF exerts diverse physiological activities including anti-angiogenesis, anti-vasopermeability, anti-tumor, and neurotrophic activities. PEDF acts via multiple high affinity ligands and cell receptors. It has been described as a natural angiogenesis inhibitor with neurotrophic and immune-modulation properties. PEDF induces macrophages apoptosis and necrosis through the activation of peroxisome proliferator-activated receptor-gamma by which PEDF could modulate inflammatory reactions in septic shock. It balances angiogenesis in the eye and blocks tumor progression.
Chemical Properties
Molecular Weight45.9 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

serpin peptidase inhibitor, clade F,member 1
Related Tags: PEDF Protein, Rat, Recombinant (His) chemical structure | PEDF Protein, Rat, Recombinant (His) molecular weight