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- Product Name
- PerCP-Cy5.5 Anti-Mouse CD45R/B220 Antibody(RA3-6B2)
- Catalogue No.
- PCP55-30159
- Form
- liquid
- Conjugation
- PerCP-Cy5.5
- Conjugation Information
- PerCP-Cy5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).
- Clonality
- monoclonal
- Isotype
- IgG2a, kappa
- Clone ID
- RA3-6B2
- Storage
- PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Alternative Names
- CD45R|B220 antibody
- UniProt ID
- Q64224
Application
- Tested Applications
- FC
- Recommended dilution
- Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Validated Images
Staining of C57BL/6 murine splenocytes cells with PerCP-Cy5.5 Anti-Mouse CD45R (B220) Antibody(RA3-6B2) (filled red histogram) or PerCP-Cy5.5 Rat IgG2a, κ Isotype Control (empty black histogram). Total viable cells were used for analysis.