Specifications

Product Data
N-Tag	His
E. coli Selection	Ampicillin (100 ug/mL)
Features	Upon IPTG induction, ORFs cloned in this vector can be expressed in E.coli (BL21/DE3) as a tagged protein with an N-terminal His tag. Detection and purification of the transgene are best done using an anti-His antibody or a nickel affinity column. PS100030_F Primer can be used to sequence the target sequence after cloning. PS100028_R Primer can be used to sequence the target sequence after cloning.
Schematic of the multiple cloning sites