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> PEX6 Antibody

PEX6 Antibody

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Référence ASM10302-STR-100

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  • ICC/IF - PEX6 Antibody ASM10302
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PEX6 Monoclonal Antibody, Clone S233-8 (ASM10302). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PEX6 Monoclonal Antibody (ASM10302) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Peroxisome, Peroxisome Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PEX6 Antibody (D) Composite.
  • WB - PEX6 Antibody ASM10302
    Western Blot analysis of Rat Brain showing detection of ~100 kDa PEX6 protein using Mouse Anti-PEX6 Monoclonal Antibody, Clone S233-8 (ASM10302). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-PEX6 Monoclonal Antibody (ASM10302) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC
Primary Accession Q13608
Other Accession NP_000278.3
Host Mouse
Isotype IgG1
Reactivity Human, Rat
Clonality Monoclonal
Description Mouse Anti-Human PEX6 Monoclonal IgG1
Target/Specificity Detects ~100kDa.
Other Names PAF 2 Antibody, Peroxin 6 Antibody, Peroxisomal AAA type ATPase 1 Antibody, Peroxisomal biogenesis factor 6 Antibody, PXAAA1 Antibody, peroxisomal-type ATPase 1 Antibody, peroxisome biogenesis factor 6 Antibody
Clone Names S233-8
Immunogen Fusion protein amino acids 2-294 (N-terminus) of human PEX6. Rat: 82% identity (243/293 amino acids identical). Mouse: 82% identity (244/294 amino acids identical). <50% identity with other PEX proteins.
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-470 was sufficient for detection of PEX6 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Peroxisome | Peroxisome Membrane