Phaseolus vulgaris Lectin (PHA-E)
Référence L1240-5
Conditionnement : 5mg
Marque : GLYcoDiag
Phaseolus vulgaris lectin E (PHA-E)
| Reference | Short Name | Common Name | Inhibitory Carbohydrate | Glycans Structures Specifities |
|---|---|---|---|---|
| L1240 | PHA-E | Phaseolus vulgaris | Gal, LacNAc | ß-D-Gal-(1-3)-D-GalNAc |
Phaseolus vulgaris lectin (PHA-E) is isolated from red kidney beans and purified by affinity chromatography and ion-exchange chromatography. It is a tetrameric protein with a molecular weight of 128 kDa. The lectin recognizes and binds specifically to terminal galactose, N- acetylglucosamine and mannose residues of complex glycans on mammalian glycoproteins (1).
Because of its strong erythrocyte agglutinating activity, it is also known as erythroagglutinin (PHA-E). PHA-E is not blood group specific (2). Less than 5 μg/mL agglutinates
human erythrocytes of type O and agglutination can be inhibited by certain oligosaccharides. PHA-E lectin is supplied as a white lyophilized powder from a buffer containing from 50 mM NH4 HCO3. The purity of the lectin in determined by SDS-PAGE, which generates one single band at 32 kDa.
Applications
Validated in GLYcoPROFILE®
Erythrocyte agglutination studies
Model system of how proteins recognize carbohydrates
Features
Ultrapure quality
Galactose-binding lectin
Specific for a tumour-associated T-antigenic disaccharide
Agglutinates human erythrocytes at a concentration of ≥ 7.8 μg/ml
Lyophilized powder
Ordering informations
| Reference | Name | Quantity |
|---|---|---|
| L1240-5 | PHA-E lectin | 5 mg |
References
(1) Chrispeels M. J., Raikhel N. V. (1991). Lectins, Lectin Genes, and Their Role in Plant Defense. The Plant Cell Vol.3, 1–9.
(2) Liener I. E., Sharon N., Goldstein I. J., (1986) The Lectins – Properties, Functions and Applications in Biology and Medicine.
| Reference | Short Name | Common Name | Inhibitory Carbohydrate | Glycans Structures Specifities | |
|---|---|---|---|---|---|
| L1222 | ABA | Agaricus Bisporus | Gal/GalNAc | Gal(β-1,3) GaINAc | |
| L1221 | AIA / Jacalin | Artocarpus intergrifolia | Gal | Galα1-6 or Galβ1-3GalNAc (T-antigen)>> lactose, more specific for T-antigen than PNA | |
| L1367 | AML | Astragalus membranaceus | Gal | Galβ | |
| L1205 | ASA | Allium sativum agglutinin | α(1,3)Man | Mannose | |
| L1889 | BanLec | Musa acuminata | Mannose, Glucose | αMan | |
| L1255 | BC2L-A | Burkholderia cenocepacia | α(1,3)Man | Mannose-containing ligands | |
| L1256 | BC2L-C | Burkholderia cenocepacia | Lewis b / Lewis Y | Fucose containing ligands | |
| L1254 | CJA | Crotalaria juncea | lactose | Gal > GalNAc | |
| L1366 | cMol | Moringa oleifera | fetuin, thyroglobuline | GlcNAc, Complex carbohydrate-binding specificity | |
| L1201 | Con A | Concanavalin A | MeαMan | α-Man, Glc |
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