Biological Activity

Phosphatidylcholine is a major constituent of cell membranes and pulmonary surfactant, and is more commonly found in the exoplasmic or outer leaflet of a cell membrane. Phosphatidylcholine also plays a role in membrane-mediated cell signaling and PCTP activation of other enzymes.

Customer Product Validations & Biological Datas

	Source	Nat Commun (2017). Figure 5. Phosphatidylcholine
	Method	Immunocytochemistry
	Cell Lines	HeLa cells
	Concentrations	5 mg/mL
	Incubation Time	30 min
	Results	Within 30 min after adding liposomes, whereas PC fluorescent signal inside the cells was weak (Fig. 5i), PS-positive vesicles were readily detected inside WT MEF.

Protocol (for reference only)

Cell Experiment
Cell lines	Chang liver cells
Preparation method	Chang liver cells were plated in a 96-well dish (six replicates per sample), then treated with ethanol for 10 h, simultaneously and after the different concentrations of phosphatidylcholine (50, 75, and 100 nmol/L) were incubated for 24 h. 50 ll of MTT (5 mg/ml in PBS) was added to each well.
Concentrations	50, 75, and 100 nmol/L
Incubation time	24 h

Animal Experiment
Animal models
Formulation
Dosages
Administration

Chemical Information

Molecular Weight	758.06
Formula	C42H80NO8P
CAS Number	8002-43-5
Solubility (25°C)	Ethanol ≥ 8 mg/mL
Storage	2-8°C, dry, protect from light, sealed

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Zhang Y, et al. J Membr Biol. Protective effect of phosphatidylcholine on restoration of ethanol-injured hepatocytes related with caveolin-1.