As a component of the Thomson Ultra Yield® system, Plasmid+® media plays a significant role in helping to generate high microbial titers resulting in higher plasmid yields.
to your microbial cultures
Key Features
Medium specifically formulated for high DNA growth
Increased supercoiled DNA per Liter
Consistent plasmid production up to 22 hours
Use with tubes, plates, flasks, and fermenters
Sterile and ready to use. Simply add antibiotics and Grow!
Store Plasmid+® liquid media at room temperature for up to 12 months
Animal Origin Free Formulation
in Plasmid Production
The following diagram illustrates the increased efficiency in plasmid production when using Plasmid+® media over conventional media. A plasmid yield of 2.5mg was achieved when using either a 2.5L Ultra Yield® flask with 500mL of conventional media or a 250mL Ultra Yield® flask with 60mL of Plasmid+® media. No need to use large flasks and lots of media when you can get by with 8x less media to generate the same results.
Please use Antifoam 1:5000 (Sigma-Aldrich® Antifoam 204)
“My plasmid maxi preps yield has improved a lot. I use QiagenR Hi-Speed maxi kits and I used to grow 250mL bacterial culture to load on one column and my purified yield would barely be around 1mg/mL. UsingPlasmid+® media I grow 50mL culture for one QiagenR Hi-Speed column and in the past few weeks I even scaled the culture to 30mL/column and my yield is between 1-1.5mg/mL.”
Thomson is not affiliated with Novartis® San Diego or its subsidiaries
for E.coli with Plasmid+® Media
Four E. coli constructs were grown in either LB media or Plasmid+® media in 250mL Ultra Yield® Flasks using optimal fill volumes and shake speeds. After 24 hours in culture each flask was harvested and plasmid yields compared. As shown, Plasmid+® media greatly improved plasmid yields in all cultures on average over 1900% compared with LB Media
Improved DNA Protocol for E.coli with Plasmid+® Media - available in Technical Resources