PMA, 20mM in H2O
Référence 40019
Conditionnement : 100UL
Marque : Biotium
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PMA (Propidium Monoazide)
A photo-reactive DNA-binding dye typically used in viability PCR (v-PCR) of microorganisms like bacteria, viruses, and fungi.
Product Attributes
| Viability dye | PMA |
|---|---|
| Molecular weight | 511 |
| Excitation/Emission | Abs = 464 nm (before photolysis), ~510/~610 nm (with DNA/RNA, after photolysis) |
| Assay type/options | Live/dead discrimination, Viability PCR |
| Storage Conditions | 1 mg solid, store at 2 to 8 °C, 100 uL in water, store at -10 to -35 °C |
| Detection method/readout | PCR/qPCR |
Technology
Microbiology Products
Viability PCR
The Full Spectrum Blog
Blog: 12 years later: Viability PCR using PMA and PMAxx™ dyes
Blog: Switching from agar plates to v-PCR for bacteria viability testing – What you need to know
Product Description
PMA (propidium monoazide) is a photo-reactive DNA-binding dye used in viability PCR (v-PCR) of microorganisms like bacteria, viruses, and fungi. PMA is available as a 1 mg lyophilized solid or 20 mM solution in water. Also try PMAxx™ (40069), a superior alternative to PMA.
- Selectively detect viable cells using qPCR
- Validated in hundreds of publications
- Dead cell specific dye, binds to DNA
- Covalently attaches to DNA after photoactivation
- Available as 1 mg lyophilized solid or 20 mM solution in water
- λAbs = 464 nm (before photolysis); λAbs/λEm= ~510/~610 nm (with DNA/RNA, after photolysis)
To learn more about the advantages of determining microbial or cell viability using viability PCR, visit the Viability PCR Technology Page.
| Product | Catalog Number | Unit Size | Format |
|---|---|---|---|
| PMA Dye | 40013 | 1 mg | Lyophilized solid |
| PMA Dye, 20 mM in H2O | 40019 | 100 uL (20 mM in water) | Solution |
About PMA
PMA dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive dye that binds to DNA with high affinity. Upon photolysis with visible light, PMA dye becomes covalently attached to DNA. This modified DNA cannot be amplified by PCR. The dye is designed to be cell membrane-impermeable. Thus, in a population of live and dead cells, only dead cells are susceptible to DNA modification due to compromised cell membranes. This unique feature of PMA dye makes it highly useful in selective detection of live bacteria by qPCR.
Since Biotium first developed PMA dye, there have been hundreds of publications on the use of the dye in many sample types including dozens of bacterial strains, biofilms, yeast, fungi, viruses, and eukaryotic cells. It has been used in such applications as food and water safety and environmental testing, and has been used in conjunction with qPCR, NextGen Sequencing (NGS), Sanger sequencing, and Loop-mediated Isothermal Amplification (LAMP).
PMAxx technology is covered by granted and/or pending US and international patents.
References
Download PMA and PMAxx™ References and a list of PMA and PMAxx™ Validated Bacterial Strains.