Polyclonal Rabbit Anti-Rat IgG Secondary Antibody (HRP, IHC, WB)

Référence LS-C70023-1

Conditionnement : 1mg

Marque : LSBio


Overview

Antibody:
Rat IgG Rabbit anti-Rat Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Rat
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
Rat IgG antibody LS-C70023 is an HRP-conjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB.
Target
Rat IgG
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Rat IgG whole molecule
Specificity
Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, rat IgG and rat Serum. No reaction was observed against human serum proteins.
Applications
  • IHC (1:500 - 1:2000)
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:2000 - 1:10000)
Usage
Suitable for use in Western Blot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and immunohistochemistry. ELISA: 1:2000-1:10000. Use 1 ug of Rat IgG in a standard capture ELISA. Western Blot: 1:1000-1:5000. Immunohistochemistry: 1:500-1:2000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/ml BSA (IgG, Protease free), PBS, pH 7.2, 0.01% Gentamicin
Reconstitution
sterile ddH2O.
Storage
May be stored at -20°C lyophilized. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C. Labeled with horseradish peroxidase (HRP). Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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