RayBio® C-Series Porcine Inflammation Array C1 Kit. Detects 26 Porcine Inflammation factors. Suitable for all liquid sample types.
Porcine Inflammation Array C1 Map
Porcine Inflammation Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Porcine (pig) |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Accession Number | Q9TU73|Q711P4|B0FYK2|Q29118|P49879|Q29412|P17803|Q29053|Q95J68|Q95253|Q60I29|F1RKV0|P18430|P26889|Q29056|K7GM74|D0FHA1|Q04745|P26893|P26894|P80928|Q29288|P23563|Q29055|Q76LU6 |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 26 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| Eotaxin-1 (CCL11)
| GM-CSF
| IFN alpha
| IFN-beta
| IFN-gamma
|
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-1 Ra (IL-1 F3)
| IL-12 p70
| |
| IL-13
| IL-15
| IL-17A
| IL-17F
| IL-18
|
| IL-22
| IL-4
| IL-6
| ||
| IL-8 (CXCL8)
| MIF
| MIG (CXCL9)
| MIP-1 beta (CCL4)
| RANTES (CCL5)
|
| TNF alpha
| ||||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Porcine Inflammation Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.