For general laboratory use.

Unit Definition: One unit is defined as the amount of enzyme required to hydrolyze casein to produce 1 μmol tyrosine per minute at 37°C and pH 8.0.

Shipping: shipped at ambient temperature

Storage Conditions: store at 4 °C

Shelf Life: 12 months

Molecular Weight: 28.9 kDa

CAS#: 39450-01-6

EC number: 254-457-8

Purity: free of RNase, DNases and Nickase

Form: amorphous powder

Color: white to off white

Activity: > 30 units/mg

Applications:
Digestion of proteins during DNA and RNA preparation.

Description:
Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The protein with a molecular weight of 28.9 kDa cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinse K is not inactivated by metal chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4 - 12.5).
Proteinase K is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF.
In presence of 0.5 - 1 % SDS Proteinase K inactivates DNases and RNases in eucaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids.

Hazard pictograms:

Signal word: Danger

Hazard statements:
H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary statements:
P304 + P340 IF INHALED: Remove person to fresh air and keep comfortable for breathing.

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