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PY-60 [2765218-56-0]

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Référence NB-64-45841-5mg

Conditionnement : 5mg

Marque : Neo Biotech

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PY-60

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Catalog No. T9566 Copy Product Info

Purity: 99.67%

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PY-60 can effectively activate YAP transcriptional activity against annexin A2 (ANXA2) (Kd value of 1.4 µM). PY-60 activates a pro-proliferative, YAP-dependent transcriptional program in adult animals that are able to remodel the epidermis through proliferation.

Cas No. 2765218-56-0

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Purity:99.67%

Appearance:Solid

Color:White

Product Information

Bioactivity

Description	PY-60 can effectively activate YAP transcriptional activity against annexin A2 (ANXA2) (Kd value of 1.4 µM). PY-60 activates a pro-proliferative, YAP-dependent transcriptional program in adult animals that are able to remodel the epidermis through proliferation.
Targets & IC50	ANXA2:1.4 µM
In vitro	METHODS: PY-60 (20µM, 24 hours) treated 293A cells, and immunoblot analysis of PP2CA-V5 was performed on material obtained by anti-FLAG immunoprecipitation from cells overexpressing ANXA2-FLAG. RESULTS In 293A cells treated with PY-60, the association between ANXA2 and PPP2CA increased significantly. [1]
In vivo	METHODS: PY-60 (5 mg/kg, intravenous injection, 24 hours) was administered to mice, and the changes in plasma levels in mice were evaluated and the intrinsic clearance in mice was measured. RESULTS The half-life (T1/2) was 0.46 h, and the AUC0-24 was 623 ng*h/mL. At the same time, PY-60 showed a rapid clearance rate (133 mL/min/kg). [2]

Chemical Properties

Molecular Weight	313.37
Formula	C₁₆H₁₅N₃O₂S
Cas No.	2765218-56-0
Smiles	C(NCCCC1=NC=CS1)(=O)C=2C=C(ON2)C3=CC=CC=C3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (319.11 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (12.76 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.1911 mL	15.9556 mL	31.9112 mL	159.5558 mL
5 mM	0.6382 mL	3.1911 mL	6.3822 mL	31.9112 mL
10 mM	0.3191 mL	1.5956 mL	3.1911 mL	15.9556 mL
20 mM	0.1596 mL	0.7978 mL	1.5956 mL	7.9778 mL
50 mM	0.0638 mL	0.3191 mL	0.6382 mL	3.1911 mL
100 mM	0.0319 mL	0.1596 mL	0.3191 mL	1.5956 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

YAPPY-60PY60PY 60AnnexinAAnnexin A2Annexin A

Related Tags: PY-60 chemical structure | PY-60 in vivo | PY-60 in vitro | PY-60 formula | PY-60 molecular weight