Pyruvate kinase (PK) is an important enzyme involved in glycolysis metabolic pathway. PK catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, producing ATP and pyruvate. Lack of pyruvate kinase will cause the disease pyruvate kinase deficiency which slows down the process of glycolysis.

 The Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit provides a sensitive, simple, fast and convenient way for accurate detection of PK activity in various samples (blood, tissues, and culture cells, etc.) based on colorimetric and fluorometric method. In the assay, PEP and ADP were catalyzed by PK to produce ATP and pyruvate, which is subsequently oxidized by pyruvate oxidase to generate fluorescence (Ex/Em = 535/587 nm) and color (λ = 570 nm). The increase in fluorescence intensity or color is proportional to the increase in pyruvate amount. The kit can detect 0.1 mU/mL pyruvate kinase.