Rat Dehydroepiandrosterone sulfate,DHEA-S ELISA Kit
Référence CSB-E05106r-96T
Conditionnement : 96T
Marque : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Dehydroepiandrosterone sulfate,DHEA-S
Abbreviation
DHEA-S
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.81 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of rat DHEA-S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 87 | ||||
| Range % | 81-92 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 91-104 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 84-98 | |||||
Recovery
| The recovery of rat DHEA-S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-97 | ||||
| EDTA plasma (n=4) | 97 | 91-101 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Rat DHEA-S ELISA Kit was designed for the quantitative measurement of Rat DHEA-S protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.
Citations
- T Zhou, Y Tan, J Chen, J Shi, Y Zhang,Scientific Reports,2026
- J Shi, Y Wang, L Li, R Wang, Y Peng, T Zhou,Ecotoxicology and Environmental Safety,2025
- A Moustafa,Free Radical Biology and Medicine,2020
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