Rat IgM (mu chain) Antibody Alkaline Phosphatase Conjugated

Référence orb347817-1mg

Conditionnement : 1mg

Marque : Biorbyt


Rat IgM (mu chain) Antibody Alkaline Phosphatase Conjugated

SKU: orb347817

Description

Rat IgM (mu chain) antibody (Alkaline Phosphatase)

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsDOT, ELISA, IHC, WB
Dilution RangeELISA: 1:10,000 - 1:30,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
ReactivityRat
Application Notes
Anti-Rat IgM Alkaline Phosphatase antibody has been tested by ELISA and dot blot and is assayed against 1.0 ug of Rat IgM in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 is suggested for this product.

Key Properties

Antibody TypeSecondary Antibody
HostGoat
ClonalityPolyclonal
IsotypeIgG
ImmunogenRat IgM whole molecule
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgM and Rat Serum. No reaction was observed against other rat heavy or light chain proteins.
ConjugationAP

Storage & Handling

StorageStore vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Concentration1.0 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

goat anti-Rat IgM (mu chain) Antibody Alkaline Phosphatase Conjugation, goat anti-Rat IgM mu chain Alkaline Phosphatase Conjugated Antibody

Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC
Immunohistochemistry
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
DOT
Dot Blot
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