The RayBiotech Annexin V Detection Kit is a sensitive assay of cell apoptosis by flow cytometry. Annexin V is a cellular protein that specifically bind to phosphatidylserine (PS) sites on cell membrane. In healthy live cells, PS is predominantly located along the cytosolic side of the plasma membrane while during apoptosis and necrosis PS translocates to the extracellular membrane, which can be detected by fluorochrome-labeled Annexin V. Live and dead cells can be discriminated by RayBright Live/dead markers, a group of small organic dye. Unlike other apoptosis assay using propidium iodide (PI) or 7-AAD, RayBio Annexin V apoptosis Detection Kit can combine with further immunophenotyping that requires cell fixation.

• RayBright® R780 Live (in DMSO, 1:100 dilution for use)
• RayBright® V450 Annexin V (5 µl/test)
• Annexin V Binding Buffer, 5x (dilute with ddH2O to 1x solution)
• Annexin V Co-staining Buffer, 5x (dilute with ddH2O to 1x solution)

Stored at 2 °C - 8 °C, protected from prolonged light exposure. DO NOT FREEZE.

One million human PBMCs were stained with RayBright® Live R780 and RayBright® V450 Annexin V in 1x Annexin V Binding Buffer.

Note: RayBright® is a trademark of RayBiotech, Inc.