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> Recombinant Human Stromal cell-derived factor 1 protein(CXCL12) (Active)

Recombinant Human Stromal cell-derived factor 1 protein(CXCL12) (Active)

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Référence CSB-AP000741HU-2ug

Conditionnement : 2ug

Marque : Cusabio


CSB-AP000741HU
Abbreviation Recombinant Human CXCL12 protein, partial (Active)
MSDS
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  • CSB-AP000741HU SDS-page

Product Details

Purity
≥97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/ml.
Target Names
Uniprot No.
Research Area
Immunology
Alternative Names
12-O-tetradecanoylphorbol 13-acetate repressed protein 1; AI174028; C-X-C motif chemokine 12; Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); Chemokine (C-X-C motif) ligand 12; Chemokine CXC motif ligand 12; cxcl12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; OTTHUMP00000019491 ; PBSF; Pre-B cell growth-stimulating factor; SCYB12; SDF 1; SDF-1; SDF-1-alpha(3-67); SDF-1a; SDF-1b; SDF1_HUMAN; SDF1A; SDF1B; Stromal cell-derived factor 1; Stromal cell-derived factor 1 delta ; Stromal cell-derived factor 1 gamma ; Stromal cell-derived factor 1a ; Stromal cell-derived factor-1 alpha ; Thymic lymphoma cell-stimulating factor; Tlsf; TLSF-a; TLSF-b; Tlsfa; Tlsfb; TPAR1
Species
Homo sapiens (Human)
Source
E.Coli
Expression Region
22-89aa
Complete Sequence
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK
Mol. Weight
8.0 kDa
Protein Length
Full Length of Mature Protein of Isoform Alpha
Tag Info
Tag-Free
Form
Liquid or Lyophilized powder
Buffer
20 mM PB pH 7.0, 130 mM NaCl
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description

CXCL12/SDF-1 is a principal ligand for CXCR4 and plays a critical role in lymphocyte trafficking, hematopoietic stem cell homing, and tumor metastasis, making validated chemotactic activity essential for meaningful experimental results. This tag-free recombinant human CXCL12 (22–89aa) reproduces the mature protein sequence and demonstrates confirmed biological activity in a chemotaxis bioassay using PHA/IL-2–activated human peripheral blood T-lymphocytes at 20–80 ng/ml, providing a suitable basis for migration assays, CXCR4 signaling pathway studies involving MAPK and PI3K/...

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Target Background

Function
Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of ...
Gene References into Functions
  1. CXCL12-positive cases exhibited shorter disease-free survival rates compared to CXCL12-negative cases.
  2. this study shows an essential role of CXCR7, together with CXCR4, in the control of normal and malignant hematopoietic cell migration and homing induced by CXCL12.
  3. CXCL12 rs1801157 is independently associated with Human papillomavirus infection and exerts influence in high grade intraepithelial lesions development
  4. silencing of CXCL12 had a protective effect on podocyte injury, which may be through inhibiting CXCL12/STAT3 signaling pathway.
  5. The CXCL12/SDF1 protein expression is a good prognostic biomarker in breast cancer.
  6. the CXCL12-CXCR4 axis promotes the migration, invasion, and EMT processes in B-CPAP cells, at least partly, by activating the NF-kappaB signaling pathway.
  7. Study shows that CXCL12 methylation-mediated epigenetic regulation of gene expression in papillary thyroid carcinoma (PTC). The study was the first to perform an reduced representation bisulfite sequencing analysis for PTC and suggested that CXCL12 may contribute to PTC development by methylation-mediated epigenetic regulation of gene expression.
  8. results demonstrate that non-oxidizable HMGB1 induce a sustained cardiac fibroblasts migration despite the redox state of the environment and by altering CXCL12/CXCR4 axis. This affects proper cardiac remodeling after an infarction.
  9. A basis for understanding how multiple elements in the sequence encoding the 3'UTR of the CXCL12 gene regulates its transcription and insights about diseases involving abnormal CXCL12alpha expression.
  10. High SDF-1 expression is associated with bladder cancer progression.

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