Orb1179013-100ug | Recombinant TNF beta (Tumor necrosis factor beta)

Recombinant TNF beta (Tumor necrosis factor beta), Human, Animal-Free

SKU: orb1179013

Recombinant TNF beta (Tumor necrosis factor beta), Human, Animal-Free

Cancer Biology; Immunology & Inflammation

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Tested Applications	Cell Culture, ELISA

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Source	Escherichia coli
Expression System	Escherichia coli
Biological Origin	Human
Biological Activity	Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is <3 pg/mL. The specific activity of recombinant human TNF beta is > 3.3x 108 IU/mg.
Reactivity	Human
Tag	His-tag at the N-terminus
Endotoxins	<0.1 EU per 1 μg of the protein by the LAL method.
Purity	>98% as determined by SDS-PAGE. Ni-NTA chromatography.
Protein Sequence	LPGVGLTPSAAQTARQHPKMHLAHSTLKPAAHLIGDPSKQNSLLWRANTDRAFLQDGFSLSNNSLLVPTSGIYFVYSQVVFSGKAYSPKATSSPLYLAHEVQLFSSQYPFHVPLLSSQKMVYPGLQEPWLHSMYHGAAFQLTQGDQLSTHTDGIPHLVLSPSTVFFGAFAL with polyhistidine tag at the N-terminus.

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Storage	Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Form/Appearance	Lyophilized
Buffer/Preservatives	The protein was lyophilized from a solution containing 20 mM sodium citrate, 0.2 MNaCl, pH 3.5.
Reconstitution	It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

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TNFSF1B, Lymphotoxin-alpha (LT-α)

NCBI Gene ID

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No NCBI Gene data available

Protein Handling and Storage Guide

Protein Handling Guide

ELISA

Enzyme-linked Immunosorbent Assay (EIA)