RIPA Lysis Buffer System
Référence sc-24948A
Conditionnement : 500ml
Marque : Santa Cruz Biotechnology
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RIPA Lysis Buffer System: sc-24948
RIPA Lysis Buffer System
Noms alternatifs:
RIPA Lysis Buffer System also known as RIPA (Radioimmunoprecipitation) Lysis Buffer System
Application:
RIPA Lysis Buffer System® is a high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
* Consulter le Certificat d'Analyses pour les données spécifiques à un lot (incluant la teneur en eau).
ACCÈS RAPIDE AUX LIENS
Informations pour la commande
Références bibliographiques
Description
Information Technique
Données de Sécurité
SDS & Certificat d’Analyses
RIPA (Radioimmunoprecipitation) Lysis Buffer System is used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA). RIPA lysis buffer has stronger denaturing capabilities than NP-40 (sc-281108) or Triton X-100 (sc-29112) and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts. RIPA Lysis Buffer System includes protease inhibitors, making it ready for use in mammalian cell and tissue lysis.
sc-24948, 50 mL - Components supplied in four vials:
VIAL 1: 50 mL 1X lysis buffer (pH 7.4 ±0.1)
VIAL 2: 500 μL (200mM) PMSF in DMSO
VIAL 3: 500 μL protease inhibitor cocktail in DMSO
VIAL 4: 500 μL (100mM) sodium orthovanadate in water
sc-24948A, 500 mL - Components supplied in four vials:
VIAL 1: 500 mL 1X lysis buffer
VIAL 2: 5 ml (200mM) PMSF in DMSO
VIAL 3: 5 ml protease inhibitor cocktail in DMSO
VIAL 4: 5 ml (100mM) sodium orthovanadate in water
sc-24948, 50 mL - Components supplied in four vials:
VIAL 1: 50 mL 1X lysis buffer (pH 7.4 ±0.1)
VIAL 2: 500 μL (200mM) PMSF in DMSO
VIAL 3: 500 μL protease inhibitor cocktail in DMSO
VIAL 4: 500 μL (100mM) sodium orthovanadate in water
sc-24948A, 500 mL - Components supplied in four vials:
VIAL 1: 500 mL 1X lysis buffer
VIAL 2: 5 ml (200mM) PMSF in DMSO
VIAL 3: 5 ml protease inhibitor cocktail in DMSO
VIAL 4: 5 ml (100mM) sodium orthovanadate in water
Utilisation :
1. Immediately prior to lysing cells, combine 10 μl PMSF solution, 10 μl sodium orthovanadate solution and 10-20 μl protease inhibitor cocktail solution per ml of 1X RIPA Lysis buffer to prepare complete RIPA.
2. Use 3 ml complete RIPA per gram of tissue
- OR 1 ml complete RIPA per 2.0x107 cells in suspension
- OR 0.6 ml complete RIPA per subconfluent monolayer on a 100 mm plate.
État Physique :
Liquid
STOCKAGE :
Store at -20° C
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
Télécharger la Fiche de Sécurité/ SDS (MSDS)
Region
Envoyer
SAMPLE Certificate of Analysis (COA)
RIPA Lysis Buffer System SAMPLE Certificate of AnalysisCERTIFICAT D'ANALYSE
Numéro de catalogue
Numéro de Lot
Envoyer
SAMPLE Certificate of Analysis (COA)
Catalog Number:sc-24948
Lot Number:SAMPLE
Product Name:RIPA Lysis Buffer System
| Test | Specification | Results |
|---|---|---|
| Appearance | RIPA Lysis Buffer: Clear, water white liquidNa-Ortho: Clear, colorless liquidPMSF: Clear, colorlessPIC: Slightly yellow liquid | |
| pH | RIPA Lysis Buffer: 7.355Na-Ortho: 10.004 | |
| Sterility | RIPA Lysis Buffer: Filtered with 0.2 um filterNa-Ortho: Filtered with 0.22 um filter |
TEST CONDITIONS
Exp. Date: 7/2/2027
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