This kit is suitable for purifying cell-free total RNA, including miRNA, from animal and human plasma and serum.
Serum/Plasma microRNA Kit
Serum/Plasma microRNA Kit
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Product Description
Specifications
| Size | 50 tests |
| Compatible Sample Types | Tissue Lysates, Cell Lysates |
| Shipping Type | Ambient temperature |
| Storage | Room temperature |
Product Features
- High-quality silica membrane ensures consistent RNA binding
- No isopropanol or ethanol precipitation steps needed
- Special lysis buffer allows larger sample volumes
- Suitable for RNAi, RT-PCR, Northern blot, and more
Introduction
Recent research on RNA interference and regulatory small RNAs has created a demand for a kit that can effectively extract RNA fragments of approximately 15-30 nucleotides (including siRNA and miRNA). However, traditional RNA extraction methods, such as silica membrane, cannot effectively adsorb and recover small RNA. Phenol/guanidine extraction and isopropanol/ethanol precipitation cannot effectively precipitate and recover small RNA. Serum/plasma samples are even more difficult to extract due to their unique characteristics. This kit uses a unique lysis buffer to rapidly lyse RNase in serum/plasma, and strong organic extraction to remove proteins and DNA. RNA, including small RNA, is adsorbed to a special silica membrane in the spin column under high ethanol concentration. A series of wash steps with specific buffers further removes impurities. Finally, a low-salt elution buffer elutes the purified RNA from the silica membrane.
Kit Components
- Lysis Buffer
- Wash Solutions
- RNase-free H2O
- Spin Column and Collection Tube
Assay Procedure Summary
- Mix lysis buffer with sample.
- Vortex to mix thoroughly.
- Add chloroform and mix again.
- Centrifuge and collect the upper aqueous phase.
- Add ethanol to the collected phase and mix.
- Load mixture onto spin column and centrifuge.
- Wash the column with Wash Solutions.
- Centrifuge to remove residual wash solution.
- Elute purified RNA with RNase-free water.
Storage/Stability
Store Lysis Buffer at 4°C and the rest of the kit components at room temperature. Stable for 6 months if stored properly.