STRO-1 Mouse Monoclonal Antibody [Clone ID: STRO-1]

Specifications

Product Data
Clone Name	STRO-1
Applications	FC
Recommended Dilution	Flow Cytometry.
Reactivities	Human
Host	Mouse
Isotype	IgM
Clonality	Monoclonal
Immunogen	Human CD34 positive bone marrow cells.
Specificity	This antibody detects the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.
Formulation	Tris buffered saline (TBS) Label: APC State: Liquid purified Ig fraction Preservative: 15 mM Sodium Azide Label: Conjugated with cross-linked Allophycocyanin (APC) under optimum conditions.
Concentration	lot specific
Purification	Size Exclusion Chromatography
Conjugation	APC
Storage	Store undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Stability	Shelf life: one year from despatch.
Background	STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.
Reference Data