Functional Assays. Immunoprecipitation. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 100 µl of whole blood or 106 cells in 100 µl. Immunohistochemistry on Frozen Sections: 1/10-1/100.
Reactivity
Monkey, Rat
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
Rat T Blasts and Erythrocytes. Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
Specificity
This antibody recognizes a constant determinant on the beta chain of the Alpha/Beta T Cell Receptor, expressed by 97% of peripheral T cells as defined by the OX-52 marker. Clone R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative. This Clone is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (Colic et al. 2010).
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