TFF3 Antibody
Référence MBS9607387-0,1mL
Conditionnement : 0.1mL
Marque : MyBiosource
MBS9607387 at 1/100 staining Human pancreatic cancer and adjacent nomal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.
Western blot analysis of Hela cell lysate, using TFF3 Antibody. The lane on the left is treated with the antigen-specific peptide.
TFF3 Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9607387Description: Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their… More details
Product Overview
Online Datasheet in PDFProduct Name
TFF3, Polyclonal Antibody
Full Product Name
TFF3 Antibody
Product Gene Name
anti-TFF3 antibody
Product Synonym Names
hITF; hP1.B; Intestinal trefoil factor; ITF; mITF; OTTMUSP00000021729; P1B; Polypeptide P1.B; TFF3; TFF3_HUMAN; TFI; TREFOIL; Trefoil factor (intestinal); Trefoil factor 3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
~1mg/ml (lot specific)
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
TFF3 peptide (MBS9626103) is used for blocking the activity of TFF3 antibody (MBS9607387)
Molecular Weight
8~25kDa
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human TFF3, corresponding to a region within the internal amino acids
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-TFF3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-TFF3 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
ELISA(peptide): 1:20000-1:40000
The optimal dilutions should be determined by the end user
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween20 at 4°C with gentle shaking overnight.
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
ELISA(peptide): 1:20000-1:40000
The optimal dilutions should be determined by the end user
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween20 at 4°C with gentle shaking overnight.
Immunohistochemistry (IHC) of anti-TFF3 antibody
MBS9607387 at 1/100 staining Human pancreatic cancer and adjacent nomal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.
Other Notes
Small volumes of anti-TFF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TFF3 purchase
MBS9607387 is an antibody targeting TFF3. Also known as TFF3 Antibody. Reactive in Human, Mouse. Host: Rabbit.
Additional Details
NCBI/Uniprot data below describe general gene information for TFF3. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
trefoil factor 3
NCBI Official Synonym Full Names
trefoil factor 3
NCBI Official Symbol
TFF3
NCBI Official Synonym Symbols
ITF; P1B; TFI
UniProt Protein Name
Trefoil factor 3
UniProt Synonym Protein Names
Intestinal trefoil factor; hITF; Polypeptide P1.B; hP1.B
Protein Family
Trefoil factor
UniProt Gene Name
TFF3
UniProt Synonym Gene Names
ITF; TFI; hITF; hP1.B
NCBI Protein Information
trefoil factor 3
NCBI Summary for TFF3
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for TFF3
Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-TFF3 antibody
| Reference Product | PubMed Publications |
|---|---|
| MUC5B antibody | >11 publications with TFF3 and MUC5B |
| FOS antibody | >4 publications with TFF3 and FOS |
| STAT6 antibody | >4 publications with TFF3 and STAT6 |
| GKN2 antibody | >3 publications with TFF3 and GKN2 |
| HIF1A antibody | >2 publications with TFF3 and HIF1A |
| CD55 antibody | >1 publications with TFF3 and CD55 |
Pathways associated with anti-TFF3 antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| HIF-1-alpha Transcription Factor Network Pathway antibodies | HIF-1-alpha Transcription Factor Network Pathway Diagram |
| IL4-mediated Signaling Events Pathway antibodies | IL4-mediated Signaling Events Pathway Diagram |
Diseases associated with anti-TFF3 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms Antibodies | >154 publications with TFF3 and Neoplasms |
| Carcinoma Antibodies | >94 publications with TFF3 and Carcinoma |
| Inflammation Antibodies | >52 publications with TFF3 and Inflammation |
| Adenocarcinoma Antibodies | >50 publications with TFF3 and Adenocarcinoma |
| Breast Neoplasms Antibodies | >34 publications with TFF3 and Breast Neoplasms |
| Hyperplasia Antibodies | >27 publications with TFF3 and Hyperplasia |
| Necrosis Antibodies | >24 publications with TFF3 and Necrosis |
| Pancreatic Neoplasms Antibodies | >18 publications with TFF3 and Pancreatic Neoplasms |
| Neoplasm Metastasis Antibodies | >16 publications with TFF3 and Neoplasm Metastasis |
| Liver Diseases Antibodies | >14 publications with TFF3 and Liver Diseases |
Organs/Tissues associated with anti-TFF3 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Muscle Antibodies | >333 publications with TFF3 and Muscle |
| Intestine Antibodies | >206 publications with TFF3 and Intestine |
| Stomach Antibodies | >151 publications with TFF3 and Stomach |
| Liver Antibodies | >38 publications with TFF3 and Liver |
| Thyroid Antibodies | >23 publications with TFF3 and Thyroid |
| Pancreas Antibodies | >22 publications with TFF3 and Pancreas |
| Brain Antibodies | >22 publications with TFF3 and Brain |
| Lung Antibodies | >15 publications with TFF3 and Lung |
| Eye Antibodies | >15 publications with TFF3 and Eye |
| Kidney Antibodies | >14 publications with TFF3 and Kidney |