THPTA [760952-88-3]
Référence CLK-1010-25
Conditionnement : 25mg
Marque : Jena Bioscience
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Tris((1-hydroxy-propyl-1H-1,2,3-triazol-4-yl)methyl)amine
For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at 4 °C
Shelf Life: 12 months after date of delivery
Molecular Formula: C18H30N10O3
Molecular Weight: 434.50 g/mol
Exact Mass: 434.25 g/mol
CAS#: 760952-88-3
Purity: ≥ 95 % (HPLC)
Form: solid
Color: off-white to grey
Solubility: water, DMSO, DMF, MeOH
Description:
THPTA is a water-soluble, very effective ligand for Cu(I)-catalyzed Alkyne-Azide click chemistry reactions (CuAAC). It serves a dual purpose: 1) Accelaration of the CuAAC reaction by maintaining the Cu(I) oxidation state of copper sources and 2) Protection of biomolecules from oxidative damage during the labeling reaction[1,2].
THPTA is a superior alternative to water-insoluble TBTA.
A stock solution can be prepared in ddH2O and subsequently be stored at -20°C. Avoid freeze-thaw cycles.
Presolski et al.[1] and Hong et al.[2] provide a general protocol for CuAAC reactions that may be used as a starting point for the set up and optimization of individual assays.
BIOZ Product Citations:
Selected References:
[1] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[2] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.