Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> TIE2 Protein, Rat, Recombinant (hFc)

TIE2 Protein, Rat, Recombinant (hFc)

Imprimer Imprimer
Identifiez-vous

Référence NB-64-57611-20ug

Conditionnement : 20ug

Marque : Neo Biotech


Remove All

TIE2 Protein, Rat, Recombinant (hFc)

(Synonyms: TEK tyrosine kinase, endothelial) Copy Product Info

Synonyms: TEK tyrosine kinase, endothelial

Catalog No. TMPY-02508 Copy Product Info
TIE2 Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 108 kDa and the accession number is A6JRG8.
Other Forms of TIE2 Protein, Rat, Recombinant (hFc):
TIE2 Protein, Human, Recombinant (hFc)
TIE2 Protein, Human, Recombinant (aa 23-745, His)
TIE2 Protein, Human, Recombinant (His & hFc)
TIE2 Protein, Human, Recombinant (His)
TIE2 Protein, Mouse, Recombinant (hFc)
TIE2 Protein, Cynomolgus, Recombinant (hFc)
TIE2 Protein, Human, Recombinant (His & GST)
TIE2 Protein, Mouse, Recombinant (aa 770-1122, His & GST)
TIE2 Protein, Rhesus, Recombinant (His)
TIE2 Protein, Human, Recombinant (His), Biotinylated
TIE2 Protein, Mouse, Recombinant (His), Biotinylated
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Bioactivity
1. Measured by its binding ability in a functional ELISA. 2. Immobilized S1h-3C-mANGPT2 at 10 μg/mL (100 μL/well) can bind ratTEK-Fc. The EC50 of ratTEK-Fc is 0.26-0.62μg/mL.
Description
TIE2 Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 108 kDa and the accession number is A6JRG8.
Species
Rat
Expression System
HEK293 Cells
TagC-hFc
Accession NumberA6JRG8
ConstructionA DNA sequence encoding the rat TEK (NP_001099207.1) extracellular domain (Met 4-Leu 743) was fused with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Ala 23
Protein Purity
> 95 % as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
SynonymsTEK tyrosine kinase, endothelial
Research Background
TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.
Chemical Properties
Molecular Weight108 kDa (predicted); 120-130 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Calculator

  • Reconstitution Calculator
  • Recombinant Protein Dilution Calculator
  • Specific Activity Calculator

Dose Conversion

Related Tags: TIE2 Protein, Rat, Recombinant (hFc) chemical structure | TIE2 Protein, Rat, Recombinant (hFc) molecular weight