Titin (TTN, TMD, Cardiomyopathy Dilated 1G (Autosomal Dominant), CMD1G, CMH9, CMPD4, Connectin, HMERF, LGMD2J, Rhabdomyosarcoma Antigen MU RMS 40.14)

Monoclonal Anti-Titin can be used for study of the elastic filaments within sarcomeric structures. It is also useful as a differentiation marker in the separation of rhabdomyosarcomas from other muscle tumors. By indirect immunofluorescence the antibody displays a typical striated staining pattern on frozen sections of chicken skeletal and cardiac muscle tissues. Stains the region of the A-I junction by indirect immunofluorescence. It shows a decoration line 0.05 mm from the end of the A band in electron micro-graphs. In immunoblotting, using total extracts of chicken breast muscle, the antibody reacts specifically with both bands of the titin double and shows no reaction with nebulin.

Suitable for use in Electron microscopy, Indirect Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Immunohistochemistry (Frozen): 1:1000-1:2000 fixed with acetone
Indirect Immunofluorescence (IC): 1:1000
Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Mab

IgG2b

9F238 (T11)

mouse

chicken

Not Determined

Supplied as a liquid, 0.09% sodium azide.

Ascites

Full length native Titin (purified) (Chicken breast muscle).

Recognizes chicken titin. Localizes (connectin) in skeletal and heart muscle of a wide variety of species from cold-blooded vertebrates to human. Does not crossreact with nebulin, smooth muscle or different non-muscle tissues and cultured cells.

human, mouse, rat, avian, fish and mammal.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.