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> Total Cholesterol (TC) Colorimetric Assay Kit

Total Cholesterol (TC) Colorimetric Assay Kit

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Référence orb1173223-48Tests

Conditionnement : 48T

Marque : Biorbyt


Total Cholesterol (TC) Colorimetric Assay Kit

SKU: orb1173223

Description

The kit provides a method for detecting TC concentration in biological samples such as serum, plasma, tissues, cells, and bacteria. In the assay, esterase catalyzes the hydrolysis of cholesterol esters to produce free cholesterol (FC) and free fatty acids (FFA).

Research Area

Metabolism Research

Images & Validation

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Total Cholesterol (TC) Colorimetric Assay Kit
Application Notes
The kit provides a simple method for detecting TC concentration in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria. In the assay, esterase catalyzes the hydrolysis of cholesterol esters to produce free cholesterol (FC) and free fatty acids (FFA). Then Cholesterol oxidase catalyzes free cholesterol (FC) to produce △4-cholestenone and H2O2. Further, H2O2, 4-aminoantipyrine and phenol can be catalyzed by peroxidase to form red quinone compounds which has a characteristic absorption peak at 500 nm. The Total Cholesterol (TC) present in the sample is proportional to the signal obtained.

Key Properties

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Storage & Handling

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StorageStorage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Expiration Date6 months from date of receipt.
Hazard InformationThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
DisclaimerFor research use only

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