TPI1 Antibody (C-term)
Référence MBS9208901-0,08mL
Conditionnement : 0.08mL
Marque : MyBiosource
Western blot analysis of lysates from A431, HepG2, LNCaP cell line and mouse testis, rat testis tissue lysates (from left to right), using TPI1 Antibody (C-term). MBS9208901 was diluted at 1:1000 at each lane.A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
TPI1 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TPI1 Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9208901Function: Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis.Background: TPI1 is an enzyme,… More details
Product Overview
Online Datasheet in PDFProduct Name
TPI1, Polyclonal Antibody
Full Product Name
TPI1 Antibody (C-term)
Product Gene Name
anti-TPI1 antibody
Product Synonym Names
Triosephosphate isomerase; TIM; Triose-phosphate isomerase; TPI1; TPI
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human, Mouse, Rat (Predicted Reactivity: Bovine, Monkey, Rabbit)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Specificity
This TPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-286 amino acids from the C-terminal region of human TPI1.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
TPI1 peptide (MBS9224942) is used for blocking the activity of TPI1 antibody (MBS9208901)
Applications Tested/Suitable for anti-TPI1 antibody
Immunohistochemistry-Paraffin (IHC-p), Flow Cytometry (FC), Western Blot (WB), ELISA (EIA)
Application Notes for anti-TPI1 antibody
WB~~1:1000
IHC-p~~1:50~100
FC~~1:10~50
IHC-p~~1:50~100
FC~~1:10~50
Western Blot (WB) of anti-TPI1 antibody
Western blot analysis of lysates from A431, HepG2, LNCaP cell line and mouse testis, rat testis tissue lysates (from left to right), using TPI1 Antibody (C-term). MBS9208901 was diluted at 1:1000 at each lane.A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
Immunohistochemistry (IHC) of anti-TPI1 antibody
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with TPI1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product References and Citations for anti-TPI1 antibody
Martins-de-Souza,D., et.al., BMC Psychiatry 9, 17 (2009)
Other Notes
Small volumes of anti-TPI1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TPI1 purchase
MBS9208901 is an antibody targeting TPI1. Also known as TPI1 Antibody (C-term). Reactive in Human, Mouse, Rat (Predicted Reactivity: Bovine, Monkey, Rabbit). Host: Rabbit.
Additional Details
NCBI/Uniprot data below describe general gene information for TPI1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
NCBI Official Full Name
triosephosphate isomerase isoform 1
NCBI Official Synonym Full Names
triosephosphate isomerase 1
NCBI Official Symbol
TPI1
NCBI Official Synonym Symbols
TIM; TPI; TPID; HEL-S-49
UniProt Protein Name
Triosephosphate isomerase
UniProt Synonym Protein Names
Triose-phosphate isomerase
UniProt Gene Name
TPI1
UniProt Synonym Gene Names
TPI; TIM
UniProt Entry Name
TPIS_HUMAN
NCBI Protein Information
triosephosphate isomerase
NCBI Summary for TPI1
This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
Show more UniProt Comments for TPI1
TPI1: Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Belongs to the triosephosphate isomerase family. 2 isoforms of the human protein are produced by alternative promoter.
Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - inositol phosphate; EC 5.3.1.1; Isomerase; Carbohydrate Metabolism - glycolysis and gluconeogenesis
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: extracellular space; nucleus; cytosol
Molecular Function: protein binding; triose-phosphate isomerase activity
Biological Process: pentose-phosphate shunt; glycolysis; multicellular organismal development; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis
Disease: Triosephosphate Isomerase Deficiency
Show more Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - inositol phosphate; EC 5.3.1.1; Isomerase; Carbohydrate Metabolism - glycolysis and gluconeogenesis
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: extracellular space; nucleus; cytosol
Molecular Function: protein binding; triose-phosphate isomerase activity
Biological Process: pentose-phosphate shunt; glycolysis; multicellular organismal development; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis
Disease: Triosephosphate Isomerase Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with anti-TPI1 antibody
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Diseases associated with anti-TPI1 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Breast Neoplasms Antibodies | >3 publications with TPI1 and Breast Neoplasms |
| Hypertension Antibodies | >2 publications with TPI1 and Hypertension |
| Anemia, Hemolytic Antibodies | >2 publications with TPI1 and Anemia, Hemolytic |
| Abnormalities, Drug-Induced Antibodies | >1 publications with TPI1 and Abnormalities, Drug-Induced |
| Weight Loss Antibodies | >1 publications with TPI1 and Weight Loss |
| Liver Diseases Antibodies | >1 publications with TPI1 and Liver Diseases |
| Kidney Diseases Antibodies | >1 publications with TPI1 and Kidney Diseases |
| Metabolism, Inborn Errors Antibodies | >1 publications with TPI1 and Metabolism, Inborn Errors |
| Anemia, Hemolytic, Congenital Nonspherocytic Antibodies | >1 publications with TPI1 and Anemia, Hemolytic, Congenital Nonspherocytic |
| Hypertrophy Antibodies | >1 publications with TPI1 and Hypertrophy |
Organs/Tissues associated with anti-TPI1 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >6 publications with TPI1 and Blood |
| Brain Antibodies | >6 publications with TPI1 and Brain |
| Muscle Antibodies | >5 publications with TPI1 and Muscle |
| Eye Antibodies | >3 publications with TPI1 and Eye |
| Prostate Antibodies | >2 publications with TPI1 and Prostate |
| Liver Antibodies | >2 publications with TPI1 and Liver |
| Kidney Antibodies | >2 publications with TPI1 and Kidney |
| Mammary Gland Antibodies | >1 publications with TPI1 and Mammary Gland |
| Testis Antibodies | >1 publications with TPI1 and Testis |
| Esophagus Antibodies | >1 publications with TPI1 and Esophagus |
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