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> Trak2 Antibody

Trak2 Antibody

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Référence ASM10315-A680-100

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  • ICC/IF - Trak2 Antibody ASM10315
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Trak2 Monoclonal Antibody, Clone S390-43 (ASM10315). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Trak2 Monoclonal Antibody (ASM10315) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Trak2 Antibody (D) Composite.
  • WB - Trak2 Antibody ASM10315
    Western Blot analysis of Rat Brain-Membrane showing detection of ~ 100 kDa TRAK2 protein using Mouse Anti-TRAK2 Monoclonal Antibody, Clone S390-43 (ASM10315). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-TRAK2 Monoclonal Antibody (ASM10315) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 100 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC
Primary Accession O60296
Other Accession NP_055864.2
Host Mouse
Isotype IgG2A
Reactivity Human, Rat
Clonality Monoclonal
Description Mouse Anti-Human Trak2 Monoclonal IgG2A
Target/Specificity Detects ~100kDa. Does not cross-react with TRAK1.
Other Names Trafficking kinesin-binding protein 2 Antibody, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein Antibody, GABA-A receptor-interacting factor 1 Antibody, O-GlcNAc transferase-interacting protein of 98 kDa Antibody, EMBL AAH60681.1 Antibody, Ensembl ENSMUSP00000027186 MGI 1918077 Antibody, Als2cr3 Antibody, Grif1 Antibody, Oip98 Antibody, KIAA0549 Antibody, TRAK2_Human Antibody
Clone Names S390-43
Immunogen Fusion protein amino acids 757-914 (C-terminus) of human TRAK2
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:100 dilution of SMC-483 was sufficient for detection of Trak2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Mitochondrion | Early Endosome