WNT10B (NM_003394) Human Tagged ORF Clone
Référence RC212840
Conditionnement : 10ug
WNT10B (NM_003394) Human Tagged ORF Clone
- TrueORF®
SKU
RC212840
WNT10B (Myc-DDK-tagged)-Human wingless-type MMTV integration site family, member 10B (WNT10B)
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | WNT10B |
| Synonyms | SHFM6; STHAG8; WNT-12 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms | Chromatograms  Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene | Plasmid Map  |
| ACCN | NM_003394 |
| ORF Size | 1167 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@clinisciences.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003394.4 |
| RefSeq Size | 2288 bp |
| RefSeq ORF | 1170 bp |
| Locus ID | 7480 |
| UniProt ID | O00744 |
| Cytogenetics | 12q13.12 |
| Domains | wnt |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway |
| MW | 43 kDa |
| Summary | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. provided by RefSeq, Jul 2008 |
| Product Manuals |
| FAQs |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC212840L1 | Lenti ORF clone of Human wingless-type MMTV integration site family, member 10B (WNT10B), Myc-DDK-tagged | 10 ug | | |
| RC212840L2 | Lenti ORF clone of Human wingless-type MMTV integration site family, member 10B (WNT10B), mGFP tagged | 10 ug | | |
| RC212840L3 | Lenti ORF clone of Human wingless-type MMTV integration site family, member 10B (WNT10B), Myc-DDK-tagged | 10 ug | | |
| RC212840L4 | Lenti ORF clone of Human wingless-type MMTV integration site family, member 10B (WNT10B), mGFP tagged | 10 ug | | |
| RG212840 | WNT10B (tGFP-tagged) - Human wingless-type MMTV integration site family, member 10B (WNT10B) | 10 ug | | |
| SC310452 | WNT10B (untagged)-Human wingless-type MMTV integration site family, member 10B (WNT10B) | 10 ug | | |
Citations
Non-Canonical Wnt Predominates in Activated Rat Hepatic Stellate Cells, Influencing HSC Survival and Paracrine Stimulation of Kupffer Cells
Corbett, Mann, Mann
PLoS ONE (2015) 10 (11), e0142794 DOI: 10.1371/journal.pone.0142794
Corbett, Mann, Mann
PLoS ONE (2015) 10 (11), e0142794 DOI: 10.1371/journal.pone.0142794
Cited by 42
AI Summary: OriGene products—specifically Wnt3a, Wnt5a, Wnt10b, LEF1, and sFRP4 constructs—were used to investigate the roles of canonical and non-canonical Wnt signaling in hepatic stellate cell activation and their effects on cell survival and paracrine signaling.
Snippet: Active β-catenin construct (S37A) was provided by D.L.Johnson, as used in Orford et al[42]. Wnt3a, Wnt5a, Wnt10b, LEF1 and sFRP4 constructs were sourced from Origene
Showing 1-1 of 1 papers.
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